{"id":393,"date":"2014-06-25T21:02:31","date_gmt":"2014-06-25T21:02:31","guid":{"rendered":"https:\/\/lipsick.su.domains\/?page_id=393"},"modified":"2014-06-28T00:30:57","modified_gmt":"2014-06-28T00:30:57","slug":"drosophila-myb","status":"publish","type":"page","link":"https:\/\/lipsick.su.domains\/?page_id=393","title":{"rendered":"Drosophila Myb"},"content":{"rendered":"

The fruit fly <\/span> Drosophila melanogaster<\/span><\/a> is widely used as a model system because of the powerful genetic, genomic, and cytological methods available in this organism, the high degree of similarity of its genes to those of humans, and the lack of redundancy in many gene families.\u00a0 The sole Drosophila Myb gene was discovered by Alisa Katzen and Mike Bishop (UC San Francisco) who then isolated and characterized two temperature-sensitive mutant alleles.\u00a0 Our laboratory later isolated two null alleles of Drosophila Myb.\u00a0 Studies of these mutants showed that aberrant cell divisions occur in the absence of Myb protein.\u00a0 Myb-deficient cells exhibit a variety of mitotic defects including abnormal chromosome condensation, chromosome number (ploidy), spindle assembly, chromosome attachment to the spindle, centrosome number, and cytokinesis.\u00a0 Myb null mutant animals die as late third instar larvae, a time during development when the imaginal discs that give rise to many adult tissues undergo rapid cell proliferation.
\n<\/span><\/span><\/p>\n

\"fly<\/a> \"pupael4\"<\/a>
\nFly life cycle image from FlyMove<\/a>.\u00a0 Mutant phenotype analysis adapted from Gary Larson<\/a>.<\/em><\/p>\n

The Drosophila Myb protein is localized within the cell nucleus and concentrates on DNA both in mitotically proliferating cells and in endocycling cells that lack an M phase.\u00a0 In mitotically cycling cells the Myb protein localizes to euchromatin (decondensed regions of the chromosomes) but not to heterochromatin (highly condensed regions of the chromosomes).<\/span><\/span><\/p>\n

\"GFP-Myb<\/a>
\nManak, et al, Nature Cell Biology 2007.<\/em><\/p>\n

We collaborated with Lolli Beall and Mike Botchan (UC Berkeley) to show that Myb is required for the normal amplification of chorion genes in ovarian follicle cells, a model for site-specific DNA replication in higher eukaryotes. However, studies using mosaic analysis and <\/span>compartment-specific rescue showed that Myb is not absolutely required for DNA replication in mitotically proliferating cells.\u00a0 The absence of Myb did result in a failure of the normal progression of chromosome condensation, in that heterochromatin but not euchromatin condensed properly.\u00a0 The cell cycle arrest that occurs in the absence of Myb can be rescued by ectopic expression of a Myb cDNA under control of the yeast GAL4 transcriptional activator.
\n<\/span><\/p>\n

\"compartment<\/a>
\nManak, et al, PNAS 2002; Manak, et al, Nature Cell Biology 2007<\/em><\/p>\n

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The fruit fly Drosophila melanogaster is widely used as a model system because of the powerful genetic, genomic, and cytological methods available in this organism, the high degree of similarity of its genes to those of humans, and the lack of redundancy in many gene families.\u00a0 The sole Drosophila Myb gene was discovered by Alisa … Continue reading Drosophila Myb<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-393","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=\/wp\/v2\/pages\/393","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=393"}],"version-history":[{"count":9,"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=\/wp\/v2\/pages\/393\/revisions"}],"predecessor-version":[{"id":431,"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=\/wp\/v2\/pages\/393\/revisions\/431"}],"wp:attachment":[{"href":"https:\/\/lipsick.su.domains\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=393"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}